BIOSPEC Sensor development for routine prediction of metal biouptake in freshwaters and soil solutions

Country: EU Projects
Start Date:           Duration: 36 months         Project Type: RTD
Contract Number: EVK1-CT-2001-00086
Organisation Type:  EC Project
Contaminated land-->Contaminants-->Heavy metals
Contaminated land-->Risk assessment-->Exposure pathways
Contaminated land-->Risk assessment-->Tools and procedures
Groundwater protection-->Monitoring-->Monitoring overview
Soil-->Soil Overview
Water resources and their management -->Stresses, quality and ecological status
Project objectives:
BIOSPEC will determine reliable and predictive quantitative parameters for assessment of the biouptake potential of trace heavy metals (Cu, Pb, Cd, Zn, Ni) 
by development of robust well-characterised sensors, which can be deployed routinely in situ by institutes monitoring water quality. This is a significant
advance over any existing methodologies, which typically involve extensive sample preparation and multi-step analyses, resulting in various operationally
defined fractions which are often meaningless or empirical at best. To achieve this objective, a systematic approach will be adopted in which well-defined
simple ligands, isolated natural ligands, soil solution samples and natural freshwaters are analysed in parallel by a range of analytical techniques in conjunction
with metal biouptake studies. Furthermore, we adopt an innovative and more appropriate basis for the classification of metal species by differentiating
the total metal in terms of dynamic (i.e. mobile and labile complexes) and free metal ion concentrations. Such an understanding of these dynamic aspects has
previously been very limited and is crucial to any real progress in the interpretation and practical utilisation of speciation data. Theoretically well-founded
dynamic models will be developed that have reliable predictive value, facilitate data interpretation, and can optimise the design and strategy of routine
monitoring measurements.
Project Summary:
In collaboration with end-users, the BIOSPEC project will compare sensors for the routine prediction of metal bio-uptake, based on in situ metal speciation 
measurements. The project addresses aims of the Water Framework Directive (2000/60/EC) to protect aquatic systems by setting environmental quality standards
for metals. The results will facilitate the implementation of this policy by formulating appropriate speciation-based parameters for heavy metals (Cu,
Pb, Cd, Zn, Ni) and by providing the tools to enable their routine measurement by end-users. Specifically, the project will: (i) determine simple predictive
quantitative parameters for assessing metal bio-uptake (ii) rigorously compare several dynamic speciation techniques (iii) assess the relative merits
of the techniques in the field (iv) assess the capabilities of the techniques to predict metal uptake by organisms under a range of natural conditions, and (v)
apply the sensors and models to routine monitoring at the river basin level.
Achieved Objectives:

Product Descriptions:
A Publication list is availalbe on the project home page 
Additional Information:

Project Resources:

Copper and zinc content of periphyton from two rivers as a function of dissolved metal concentration.

Interactions Between Soil Particles and Microorganisms.

AGNES: a new electroanalytical technique for measuring free metal ion concentration.

Failure of the biotic ligand and free-ion activity models to explain zinc bioaccumulation by Chlorella kesslerii.

Diffusion coefficients of humic substances in water and through agarose gel.

Complexation of copper by zwitterionic aminosulfonic (Good) buffers.

Measuring and modeling zinc and cadmium binding by humic acid

Physicochemical Kinetics and Transport at Biointerfaces.

Binding curve from normalised limiting currents of labile heterogeneous metal-macromolecular systems. The case of Cd/humic acid.

Lability and mobility effects on mixtures of ligands under steady-state conditions

The impact of the transient uptake flux on bioaccumulation. Linear adsorption and first-order internalisation coupled with spherical semi-infinite mass transport

Complexation isotherms in metal speciation studies at trace concentration levels voltammetric techniques in environmental samples.

Affinity distribution functions in multicomponent heterogeneous adsorption. Analytical inversion of isotherms to obtain affinity spectra

Some fundamental (and often overlooked) considerations underlying the free ion activity and biotic ligand modes.

Discriminating between intra- and extracellular metals using chemical extractions.

Metal speciation dynamics and bioavailability. Zn(II) and Cd(II) uptake by mussel (Mytilus edulis) and carp (Cyprinus carpio).

Stripping chronopotentiometry for metal ion speciation analysis at a microelectrode.

Stripping chronopotentiometry at scanned deposition potential (SSCP). Part 3. Irreversible electrode reactions.

Stripping chronopotentiometry at scanned deposition potential (SSCP). Part 4. The kinetic current regime.

Effect of pH on Pb uptake by the freshwater alga, Chlorella kesslerii.

Theoretical and analytical characterisation of a flow-through permeation liquid membrane with controlled flux for metal speciation measurements.

Stripping chronopotentiometry at scanned deposition potential (SSCP). Part 2. Determination of metal ion speciation parameters.

Interpretation of humic acid coagulation and soluble soil organic matter using a calculated electrostatic potential.

Aluminum speciation in natural waters: measurement using Donnan membrane technique and modeling using NICA-Donnan

Phytotoxicity and bioavailability of nickel: chemical speciation and bioaccumulation.

Physicochemical mechanisms of trace metal bioaccumulation by microorganisms

Evaluation of the Kouteckı-Koryta approximation for voltammetric currents generated by metal complex systems with various labilities.

Elementary features of stripping chronopotentiometry in the complete depletion regime.

Effects of adsorption in stripping chronopotentiometric metal speciation analysis.

Transport of humic and fulvic acids in relation to metal mobility in a copper-contaminated acid sandy soil.

A review of competitive ligand-exchange / voltammetric methods for speciation of trace metals in freshwater.

Electrochemical metal speciation analysis of chemically heterogeneous samples: the outstanding of stripping chronopotentiometry at scanned deposition potential.

Metal speciation dynamics and bioavailability: bulk depletion effects.

Role of fulvic acid on lead bioaccumulation to Chlorella kessleri.

Cd bioaccumulation by a gram negative freshwater bacterium (Rhodospirillum rubrum)

Depletive stripping chronopotentiometry: a major step forward in electrochemical stripping techniques for metal ion speciation analysis

Complexation with dissolved organic matter and solubility control of heavy metals in a sandy soil.

Understanding the effects of soil characteristics on phytotoxicity and bioavailability of nickel using speciation models.

Voltammetry of heterogeneous labile metal-macromolecular systems for any ligand-to-metal ratio. Part III. Rigorous computation of the binding curve from the normalised limiting currents.

Numerical simulations of linear scan anodic stripping voltammetry at a modified square array of hemispherical microelectrodes located in a thin-layer cell.

Physicochemistry of Pb accumulation by Chlorella vulgaris.

Permeation liquid membrane as a tool for the monitoring of bioavailable Pb in natural waters

Significance of wave form parameters in stripping chronopotentiometric metal speciation analysis.

Critical evaluation of physicochemical parameters and processes for modelling the biological uptake of trace metals in environmental (aquatic) systems
Funding Programme(s): 
EC Framework Programme 5
Link to Organisations:

Queen's University Belfast
Submitted by: EUGRIS Team Dr Stefan Gödeke  Who does what?  30/03/2005 13:04:00
Updated by: EUGRIS Team Professor Paul Bardos  Who does what?  29/09/2006 15:52:00