Title: Monitoring of the Tichá Orlice river loading by xenoestrogenic substances by means of induction of vitellogenesis in male fish 
Resource Type: document --> technical publication --> proceedings / conference paper(s) 
Country: EU Projects 
Year of publication: 2003 
Availability: Kolářová, J. et al. (2003) Monitoring of the Tichá Orlice river loading by xenoestrogenic substances by means of induction of vitellogenesis in male fish. Persistent Toxic Substances Contamination of the European Region as a reflection of results of the GEF/UNEP Regional Based Assessment of Persistent Toxic Substances (RBA PTS) in Europe, the implementation of the Stockholm Convention and 'Community Strategy for Dioxins, Furans and PCBs 
Author 1/Producer: Kolářová, J. 
Other Authors/Producers: Svobodová, Z., Žlábek, V., Hajšlová, J. 
Author / Producer Type: EC Project 
Report / download web link (=direct link): http://www.recetox.muni.cz/coe/sources/workshop_1_rba_pts/VI ...  
EUGRIS Keyword(s): Diffuse pollution-->Contaminants-->Persistent Organic Pollutants
 
Short description: The induction of vitellogenesis in male fish, which can be diagnosed also as a vitellogenin content in blood plasma, is a significant biological marker of surface waters loading by substances with estrogenic effect. Contaminated and control sites on the Tichá Orlice river were studied during the monitoring of surface waters in the Czech Republic. Blood sampling (from v. caudalis), centrifugation and deep freezing of blood plasma were performed in situ on monitored sites. Vitellogenin concentration in trout (S. trutta) blood plasma was assessed using ELISA test Rainbow trout vitellogenin EIA kit (Biosense Laboratories, Norway). Samples of fish tissues and bottom sediments for chemical analyses and evaluation of PCB, chlorinated pesticides and PAH concentrations (in bottom sediments) were collected on monitored sites. 
Link to Project(s): RECETOX Centre of Excellence in Environmental Chemistry and Ecotoxicology
 
Submitted By: Dr Stefan Gödeke WhoDoesWhat?      Last update: 14/02/2006