Title: Novel retionoid-specific luciferase reporter gene bioassay for screening of embryotoxic potential 
Resource Type: document --> technical publication --> proceedings / conference paper(s) 
Country: EU Projects 
Year of publication: 2003 
Availability: Bláha, L., Janoąek, J. et al. (2003) Novel retionoid-specific luciferase reporter gene bioassay for screening of embryotoxic potential. Persistent Toxic Substances Contamination of the European Region as a reflection of results of the GEF/UNEP Regional Based Assessment of Persistent Toxic Substances (RBA PTS) in Europe, the implementation of the Stockholm Convention and 'Community Strategy for Dioxins, Furans and PCBs 
Author 1/Producer: Bláha, L. 
Other Authors/Producers: Janoąek, J. 
Author / Producer Type: EC Project 
Report / download web link (=direct link): http://www.recetox.muni.cz/coe/sources/workshop_1_rba_pts/VI ...  
Format (e.g. PDF): PDF 
EUGRIS Keyword(s): Diffuse pollution-->Contaminants-->Persistent Organic Pollutants
 
Short description: Embryotoxicity of several persistent organic polutants (POPs) is documented from laboratory experiments and field studies. However, biochemical and cellular events involved in the POPs-induced effects (e.g. changes in retinoid concentrations) are not characterized in details. In the present study we introduce a novel in vitro bioassay with embryonal P19 cells (clone P19/A15) stably transfected with firefly luciferase gene under control of RARE (Retinoic Acid Responsive Element). Expression of the reporter luciferase activity is induced after exposure of the cells to retinoic acid (ATRA; 10-9 mol.l-1 concentrations). Simultaneous treatments by ATRA (20 nM) and picomolar concentrations of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) resulted in the substantial decrease of luciferase activity indicating selective disruption of retinoid signalling. Additional experiments with DELOR106 (commercial mixture of polychlorinated biphenyls, PCBs) and Nibrenwax (mixture of polychlorinated naphthalenes, PCNs) reveal a suitability of the bioassay for rapid screening and detection of xenobiotic-induced biochemical effects directly related to cellular differentiation, embryotoxicity and developmental toxicity. 
Link to Project(s): RECETOX Centre of Excellence in Environmental Chemistry and Ecotoxicology
 
Submitted By: Dr Stefan Gödeke WhoDoesWhat?      Last update: 14/02/2006