Title: Determination of estrogens and progestogens by mass spectrometric techniques (GC/MS, LC/MS and LC/MS/MS) 
Resource Type: document --> technical publication --> journal article 
Country: EU Projects 
Year: 2003 
Availability: Díaz-Cruz, Silvia et al. (2003) Determination of estrogens and progestogens by mass spectrometric techniques (GC/MS, LC/MS and LC/MS/MS) J. Mass Spectrom. 2003; 38: 917–923 
Author 1/Producer: Díaz-Cruz, Silvia 
Other Authors/Producers: López de Alda, Maria J. López, R. Barceló, D. 
Author / Producer Type: EC Project 
Article Weblink (=direct link): http://projects.dhi.dk/artdemo/Publications/Silvia%20Diaz%20 ...  
Format (e.g. PDF): PDF 
EUGRIS Keyword(s): Contaminated land-->Contaminants-->Contaminants overview
Short description: Steroid sex hormones and related synthetic compounds have been shown to provoke alarming estrogenic effects in aquatic organisms, such as feminization, at very low concentrations (ng/L or pg/L). In this work, different chromatographic techniques, namely, gas chromatography/mass spectrometry (GC/MS), liquid chromatography/mass spectrometry (LC/MS) and liquid chromatography/tandemmass spectrometry (LC/MS/MS), are discussed for the analysis of estrogens, both free and conjugated, and progestogens, and the sensitivities achieved with the various techniques are inter-compared. GC/MS analyses are usually carried out after derivatization of the analytes with bis(trimethylsilyl)trifluoroacetamide (BSTFA). For LC/MS and LC/MS/MS analyses, different instruments, ionization techniques (electrospray (ESI) and atmospheric pressure chemical ionization (APCI)), ionization modes (negative ion (NI) and positive ion (PI)) and monitoring modes (selected ion monitoring (SIM) and selected reaction monitoring (SRM)) are generally employed. Based on sensitivity and selectivity, LC/ESI-MS/MS is generally themethod of choice for determination of estrogens in the NI mode and of progestogens in the PI mode (instrumental detection limits (IDLs) 0.1–10 ng/mL). IDLs achieved by LC/ESI-MS in the SIM mode and by LC/ESI-MS/MS in the SRM mode were, in general, comparable, although the selectivity of the latter is significantly higher and essential to avoid false positive determinations in the analysis of real samples. Conclusions and future perspectives are outlined. 
Link to Project(s): ARTDEMO Artificial recharge demonstration project
Submitted By: Dr Stefan Gödeke WhoDoesWhat?      Last update: 14/02/2006

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