Title: Effects of 17ß-estradiol exposure in the mussel Mytilus galloprovincialis. 
Resource Type: document --> technical publication --> journal article 
Country: EU Projects 
Year: 2004 
Availability: Janer G, Lavado R, Thibaut R, Porte C (2004): Effects of 17ß-estradiol exposure in the mussel Mytilus galloprovincialis. Mar. Environ. Res. 58, 443-446 
Author 1/Producer: Janer G 
Other Authors/Producers: Lavado R, Thibaut R, Porte C 
Author / Producer Type: EC Project 
Article Weblink (=direct link): http://www.comprendo-project.org/_files/MER2004E2mussels.pdf  
Format (e.g. PDF): PDF 
EUGRIS Keyword(s): Contaminated land-->Contaminants-->Contaminants overview
Short description: Mussels Mytilus galloprovincialis were exposed to different concentrations of estradiol (20, 200, and 2000 ng/l) in a semi-static regime (1-day dosing intervals) for up to 7 days in an attempt to see how mussels dealt with exogenous estrogenic compounds. Sex hormone levels were determined in whole tissue. Free-estradiol was only significantly elevated at the highest exposure dose (up to 10-fold). Most of the estradiol was in the tissues as fatty acid esters (>78%), which sharply increased in a dose-dependent manner (from 4 ng/g in controls to 258 ng/g at the high exposure group). In contrast, neither free nor esterified testosterone levels showed significant differences between control and exposure groups. The results suggest the existence of mechanisms that allow mussels to maintain their hormonal status, and the important role that fatty acid esterification may play within those mechanisms. Synthesis and conjugation rates of estradiol were further investigated by measuring the activity of P450 aromatase, and palmitoyl-CoA:estradiol acyltransferase, in digestive gland microsomal fractions. Overall, the study contributes to the better knowledge of molluscan endocrinology, and defines new mechanisms of regulation of free steroid-levels in mussels. 
Link to Project(s): COMPRENDO Comparative Research on Endocrine Disrupters - Phylogenetic Approach and Common Principles focussing on Androgenic/Antiandrogenic Compounds
Submitted By: Dr Stefan Gödeke WhoDoesWhat?      Last update: 14/02/2006